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Characteristics of recombinant proteins used in this study.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: Characteristics of recombinant proteins used in this study.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Recombinant, Sequencing, Expressing, Variant Assay

gp120 proteins of HIV-1 Group M Subtype A and Subtype B differentially affect prostasin, MMP-2, and ErbB3 expression. Human pulmonary artery endothelial cells were treated with gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. ( A ) Prostasin was found to be higher in gp120B-treated cells, while neighboring E-selectin spots were unchanged. ( B ) MMP-2 was found to be higher in gp120B-treated cells, while neighboring progranulin spots were unchanged. ( C ) ErbB3 was found to be higher in gp120B-treated cells, while neighboring Dkk-1 spots were unchanged. Representative images are shown at the top. Densitometry values from two spots from each array were averaged, and statistical analysis was performed using results from three separate treatments/arrays. Bar graphs represent means ± SEM (N = 3 for all groups). * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: gp120 proteins of HIV-1 Group M Subtype A and Subtype B differentially affect prostasin, MMP-2, and ErbB3 expression. Human pulmonary artery endothelial cells were treated with gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. ( A ) Prostasin was found to be higher in gp120B-treated cells, while neighboring E-selectin spots were unchanged. ( B ) MMP-2 was found to be higher in gp120B-treated cells, while neighboring progranulin spots were unchanged. ( C ) ErbB3 was found to be higher in gp120B-treated cells, while neighboring Dkk-1 spots were unchanged. Representative images are shown at the top. Densitometry values from two spots from each array were averaged, and statistical analysis was performed using results from three separate treatments/arrays. Bar graphs represent means ± SEM (N = 3 for all groups). * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Expressing

Characteristic of the effects of gp120 proteins of HIV-1 Group M Subtype A and Subtype B on the prostasin expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. Bar graphs represent means ± SEM (N = 3 for all groups) of ( A ) prostasin expression, ( B ) E-selectin expression, and ( C ) the ratio of prostasin to E-selectin expression. * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: Characteristic of the effects of gp120 proteins of HIV-1 Group M Subtype A and Subtype B on the prostasin expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. Bar graphs represent means ± SEM (N = 3 for all groups) of ( A ) prostasin expression, ( B ) E-selectin expression, and ( C ) the ratio of prostasin to E-selectin expression. * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Expressing

Characteristic of the effects of gp120 proteins of HIV-1 Group M Subtype A and Subtype B on the MMP-2 expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. Bar graphs represent means ± SEM (N = 3 for all groups) of ( A ) MMP-2 expression, ( B ) progranulin expression, and ( C ) the ratio of MMP-2 to progranulin expression. * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: Characteristic of the effects of gp120 proteins of HIV-1 Group M Subtype A and Subtype B on the MMP-2 expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. Bar graphs represent means ± SEM (N = 3 for all groups) of ( A ) MMP-2 expression, ( B ) progranulin expression, and ( C ) the ratio of MMP-2 to progranulin expression. * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Expressing

Characteristic of the effects gp120 proteins of HIV-1 Group M Subtype A and Subtype B on the ErbB3 expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. Bar graphs represent means ± SEM (N = 3 for all groups) of ( A ) ErbB3 expression, ( B ) Dkk-1 expression, and ( C ) the ratio of ErbB3 to Dkk-1 expression. * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: Characteristic of the effects gp120 proteins of HIV-1 Group M Subtype A and Subtype B on the ErbB3 expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. Bar graphs represent means ± SEM (N = 3 for all groups) of ( A ) ErbB3 expression, ( B ) Dkk-1 expression, and ( C ) the ratio of ErbB3 to Dkk-1 expression. * p < 0.05. ** p < 0.01. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Expressing

gp120 proteins of HIV-1 Group M Subtype A and Subtype B differentially affect MCP-2, and MCP-3 expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. ( A ) MCP-2 was found to be higher in gp120A-treated cells. ( B ) MMP-3 was found to be higher in gp120A-treated cells. Representative images are shown at the top. Densitometry values from two spots from each array were averaged, and statistical analysis was performed using results from three separate treatments/arrays. Bar graphs represent means ± SEM (N = 3 for all groups). * p < 0.05. ** p < 0.01. The y -axis indicates the mean pixel density of protein spots.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: gp120 proteins of HIV-1 Group M Subtype A and Subtype B differentially affect MCP-2, and MCP-3 expression. Human pulmonary artery endothelial cells were treated with SARS-CoV-2 spike proteins S1, Omicron S1, and gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h in triplicate. Expression patterns of various proteins were monitored using R&D Human XL Oncology Array. ( A ) MCP-2 was found to be higher in gp120A-treated cells. ( B ) MMP-3 was found to be higher in gp120A-treated cells. Representative images are shown at the top. Densitometry values from two spots from each array were averaged, and statistical analysis was performed using results from three separate treatments/arrays. Bar graphs represent means ± SEM (N = 3 for all groups). * p < 0.05. ** p < 0.01. The y -axis indicates the mean pixel density of protein spots.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Expressing

gp120 proteins of HIV-1 Group M Subtype A and Subtype B differentially affect TARC expression. Human pulmonary artery endothelial cells were treated with gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h. Expression patterns of various proteins were monitored using R&D Human XL Cytokine Array. TARC expression was found to be higher in gp120A-treated cells, while neighboring angiopoietin-1 spots were unchanged. Representative images are shown at the top. Densitometry values from two spots from each array were averaged, and statistical analysis was performed using results from three separate treatments/arrays. Bar graphs represent means ± SEM (N = 3 for gp120A and gp120B groups; N = 2 for untreated control). * p < 0.05. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Journal: International Journal of Molecular Sciences

Article Title: gp120 Envelope Glycoproteins of HIV-1 Group M Subtype A and Subtype B Differentially Affect Gene Expression in Human Vascular Endothelial Cells

doi: 10.3390/ijms24043536

Figure Lengend Snippet: gp120 proteins of HIV-1 Group M Subtype A and Subtype B differentially affect TARC expression. Human pulmonary artery endothelial cells were treated with gp120 of HIV-1 Subtype A (gp120A) or Subtype B (gp120B) at 1 nM for 20 h. Expression patterns of various proteins were monitored using R&D Human XL Cytokine Array. TARC expression was found to be higher in gp120A-treated cells, while neighboring angiopoietin-1 spots were unchanged. Representative images are shown at the top. Densitometry values from two spots from each array were averaged, and statistical analysis was performed using results from three separate treatments/arrays. Bar graphs represent means ± SEM (N = 3 for gp120A and gp120B groups; N = 2 for untreated control). * p < 0.05. *** p < 0.001. The y -axis indicates the mean pixel density of protein spots.

Article Snippet: Cells were treated in triplicate with either the recombinant gp120 of HIV-1 Group M Subunit A (catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (catalog number 40404-V08H, Sino Biological), S1 of SARS CoV-2 spike protein (catalog number 40591-V08H, Sino Biological), or S1 of the omicron variant SARS CoV-2 spike protein (catalog number 40591-V08H41, Sino Biological).

Techniques: Expressing